F3418
Sigma
Monoclonal Anti-Cytokeratin, pan−FITC antibody produced in mouse
clone C-11, purified immunoglobulin, buffered aqueous solution
| Synonym: | Monoclonal Anti-Pan Cytokeratin |
Description
| Immunogen | keratin-enriched preparation from cultured human epidermoid carcinoma cell line A431. |
| Specificity | The antibody recognizes human cytokeratins 4, 5, 6, 8, 10, 13, and 18. A broad spectrum antibody that reacts with a wide variety of normal, reactive, and neoplastic epithelial tissues. This antibody may be used for staining cultured epithelial cell lines. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | C-11 (monoclonal) |
| form | buffered aqueous solution |
| species reactivity | bovine, frog, human, rat, kangaroo rat, mouse |
| storage condition | protect from light |
| application(s) | direct immunofluorescence: 1:50 using PtK2 cells |
| flow cytometry: 1:500 using PtK2 cells | |
| immunoblotting: suitable | |
| isotype | IgG1 |
| Gene Information | human ... KRT13(3858), KRT18(3875), KRT4(3851), KRT5(3852), KRT6A(3868), KRT6B(3854), KRT8(3856) |
| rat ... Krt1-18(706059), Krt10(450225), Krt2-5(369017), Krt2-8(25626) | |
| mouse ... Krt10(16661), Krt13(16663), Krt18(16668), Krt4(16682), Krt5(110308), Krt6a(16687), Krt6b(16688), Krt8(16691) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | O’Hare, M., et al., Potential biological role of transforming growth factor-beta1 in human congenital kidney malformations. Am. J. Pathol. 157, 1633-1647, (2000) Abstract |
| Fogo, A., et al., αvβ6 Integrin Regulates Renal Fibrosis and Inflammation in Alport Mouse Am. J. Pathol. 170, 110-125, (2007) Abstract | |
| Fine, D.H., et al., The Actinobacillus actinomycetemcomitans Autotransporter Adhesin Aae Exhibits Specificity for Buccal Epithelial Cells from Humans and Old World Primates Infect. Immun. 73, 1947-1953, (2005) Abstract | |
| Bartek, J., et al. J. Pathol. 164, 215, (1991) | |
| Bartkova, J., et al. Neoplasma 38, 439, (1991) | |
| Lane, E.B. and Alexander, C.M. Semin. Cancer Biol. 1, 165, (1990) |
